| Product Name | MAGI3 antibody [SLP-32] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAGI3 antibody [SLP-32]MAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival. |
| Immunogen | Synthetic peptide, corresponding to C-term amino acids 421-785 of Rat MAGI3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | SLP-32 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1-2 μg/ml. Detects a band of approximately 160 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Slipr |
|---|---|
| Target | MAGI3 |
| Tissue Specificity | MAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival. |
| Cellular Localization | Cell Membrane |
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1-2 μg/ml. Detects a band of approximately 160 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS |
