Application
Recombinant protein MBP-tagged BCDEF, TPD52 and MAGEA3(each 20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL| Product Name | Maltose binding protein antibody [10C12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10C12 ] to MBPEpitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. |
| Immunogen | Recombinant MBP purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10C12 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB-Ag: Use at 1:1,000 ~ 2,000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Maltose binding protein |
|---|---|
| Target | MBP |
| Light Chain Type | kappa |
Application
Recombinant protein MBP-tagged BCDEF, TPD52 and MAGEA3(each 20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL| Application Notes | WB-Ag: Use at 1:1,000 ~ 2,000. Optimal working concentrations should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
