Application
ICC/IF analysis of HeLa cells using MAP2 antibody at a dilution of 1:150.Application
WB analysis of mouse brain and HeLa lysates using MAP2 antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysates using control IgG and MAP2 antibody. The precipitates were detected by the same MAP2 antibody.| Product Name | MAP2 antibody [1D7-B9-C8-F6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1D7-B9-C8-F6] to MAP2This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010] |
| Immunogen | Purified recombinant MAP2 protein fragments expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1D7-B9-C8-F6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000. For ICC/IF: Use at a dilution of 1:150. Optimal dilutions should be determined experimentally by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAP2B , MAP2C , microtubule-associated protein 2 , MAP2A |
|---|---|
| Target | MAP2 |
| Molecular Weight(MW) | 300kDa (note) |
| Cellular Localization | Cytoplasm ＞cytoskeleton |
Application
ICC/IF analysis of HeLa cells using MAP2 antibody at a dilution of 1:150.
Application
WB analysis of mouse brain and HeLa lysates using MAP2 antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysates using control IgG and MAP2 antibody. The precipitates were detected by the same MAP2 antibody.| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000. For ICC/IF: Use at a dilution of 1:150. Optimal dilutions should be determined experimentally by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150mM NaCl)with 0.2%sodium azide,0.1%BSA,50%glycerol. |
