Application
Staining of primary cortical neuronal culture using anti-MAP2 antibody (green) at a 1:200 dilution and counterstained with DAPI (blue).Application
UCC/IF analysis of SH-SY-5Y cells using MAP2 antibody [AP20] at a dilution of 1:500 (red) and DAPI (blue).Application
Rat brain cortex whole cell extracts were separated on SDS-PAGE, blotted with Monoclonal Anti-MAP2 (2A+2B) and developed using Goat Anti-Mouse IgG, Alkaline Phosphatase and NBT/BCIP substrate. (Lane A) Antibody dilution 1:500; (Lane B) Negative control (only secondary antibody).| Product Name | MAP2 antibody [AP20] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AP - 20] to MAP2MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. |
| Immunogen | Bovine protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AP20 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For ICC: Use at a 1:200 dilution. | |
| Species Reactivity | MouseRatQuailSalamanderXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Dendrite specific MAP, MAP2A, MAP2B, MAP2C, Microtubule associated protein 2, Mtap 2 |
|---|---|
| Target | MAP2 |
| Tissue Specificity | The antibody localizes the high molecular weight forms of MAP2 namely MAP2a and MAP2b, but shows no reactivity with MAP2c. No cross-reactivity is observed with MAP1, MAP5, tubulin, or tau. |
| Cellular Localization | Cytoplasmic |
Application
Staining of primary cortical neuronal culture using anti-MAP2 antibody (green) at a 1:200 dilution and counterstained with DAPI (blue).
Application
UCC/IF analysis of SH-SY-5Y cells using MAP2 antibody [AP20] at a dilution of 1:500 (red) and DAPI (blue).
Application
Rat brain cortex whole cell extracts were separated on SDS-PAGE, blotted with Monoclonal Anti-MAP2 (2A+2B) and developed using Goat Anti-Mouse IgG, Alkaline Phosphatase and NBT/BCIP substrate. (Lane A) Antibody dilution 1:500; (Lane B) Negative control (only secondary antibody).| Application Notes | For ICC: Use at a 1:200 dilution. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide |
