Application
Immunofluorescent staining of HT29 cells using anti-MAP2K1 mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MAP2K1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. ( Dilution 1:50)Application
Anti-MAP2K1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MAP2K1Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-MAP2K1 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1 .| Product Name | MAP2K1 antibody [1C1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C1] to MAP2K1Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. |
| Immunogen | Protein expressed in 293T cell transfected with human MAP2K1 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1C1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKMK1, MAP2K1, MKK1, MAPKK1, MEK1 |
|---|---|
| Target | MAP2K1 |
| Tissue Specificity | is specific for human MAP2K1. |
Application
Immunofluorescent staining of HT29 cells using anti-MAP2K1 mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded colon tissue using anti-MAP2K1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-MAP2K1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MAP2K1
Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-MAP2K1 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1 .| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
