Western blot analysis using anti-MAP4K4 monoclonal antibody against truncated Trx-MAP4K4 recombinant protein(1), MBP-MAP4K4(aa300-400) recombinant protein(2) and MAP4K4(aa194-436)-hIgGFc transfected CH0-K1 cell lysate(3).
Mouse monoclonal antibody [3C7B5] to MAP4K4The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Immunogen
The immunogen corresponding to a region within amino acids 400 and 500 of MAP4K4 expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C7B5
Host Species
Mouse
Tested Applications
ELISAWB
ELISA: 1/10000.
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
MEKKK4 , NIK , HGK , FLH21957
Target
MAP4K4
Application
Application
Western blot analysis using anti-MAP4K4 monoclonal antibody against truncated Trx-MAP4K4 recombinant protein(1), MBP-MAP4K4(aa300-400) recombinant protein(2) and MAP4K4(aa194-436)-hIgGFc transfected CH0-K1 cell lysate(3).
Application Notes
ELISA: 1/10000.
Additional Information
Form
Liquid
Storage Instructions
Store at 4℃, for long term storage, store at -20℃.