| Product Name | MAPKAPK-2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MAPKAPK-2MAP kinase-activated protein kinase-2 (MAPKAPK-2), also known as p45 hsp27 kinase, is a 45-54 kDa serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MAPKAPK-2 is activated in response to stress, IL-1 and TNF, possibly catalyzed by p38/Hog-dependent phosphorylation. One of the major substrates of MAPKAPK-2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blot analysis (0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually. The affinity purified antibody detects both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.: |
|
| Species Reactivity | HumanMouseDogRatBovine |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MK2 MAPKAPK2 |
|---|---|
| Target | MAPKAPK-2 |
| Tissue Specificity | This antibody is specific for MAPKAPK-2. |
| Application Notes | Western blot analysis (0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually. The affinity purified antibody detects both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
