Application
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS Antibody .Application
Western blot analysis of extracts from 3T3 cells using MARCKS Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from 3T3 cells using MARCKS(Ab-158) Antibody and the same antibody preincubated with blocking peptide| Product Name | MARCKS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MARCKSMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| Immunogen | Peptide sequence around aa.156~160 (R-F-S-F-K) derived from Human MARCKS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL, MARCKS, phosphomyristin, protein kinase C substrate, 80 kDa protein, light chain, myristoylated alanine-rich C-kinase substrate, myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L), myristo |
|---|---|
| Target | MARCKS |
| Molecular Weight(MW) | 80 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS Antibody .
Application
Western blot analysis of extracts from 3T3 cells using MARCKS Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from 3T3 cells using MARCKS(Ab-158) Antibody and the same antibody preincubated with blocking peptide| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
