Rabbit Polyclonal antibody to MARCKSMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Immunogen
Peptide sequence around aa. 160~164 (K-K-S-F-K) derived from Human MARCKS.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: , ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL, MARCKS, phosphomyristin, protein kinase C substrate, 80 kDa protein, light chain, myristoylated alanine-rich C-kinase substrate, myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L), myristo
Target
MARCKS
Molecular Weight(MW)
80 (note)
Application
Application
Western blot analysis of extracts from Hela and HT29 cells using MARCKS Antibody .
Application
Western blot analysis of extracts from Hela and HT29 cells using MARCKS(Ab-162) Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: , ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.