Application
Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MASP1 (heavy chain,Cleaved-Arg448) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MASP1 (cleaved Arg448) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MASP1 (cleaved Arg448)Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MASP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MASP DKFZp686I01199 MASP1 CRARF1 MASP3 CRARF PRSS5 MGC126283 MAP1 RaRF FLJ26383 MGC126284 MAp44 MASP1 (cleaved R448) MASP1 (cleaved Arg448) MASP1 cleaved R448 MASP1 cleaved Arg448 Cleaved MASP1 (R448) Cleaved MASP1 (Arg448) |
|---|---|
| Target | MASP1 |
| Molecular Weight(MW) | 47 kDa (note) |
| Tissue Specificity | MASP1 (heavy chain,Cleaved-Arg448) Antibody detects endogenous levels of fragment of activated MASP1 resulting from cleavage adjacent to Arg448. |
Application
Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MASP1 (heavy chain,Cleaved-Arg448) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
