WB
Western blot analysis of MASP1 using anti-MASP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MASP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of PC-3 cells using anti-MASP1 antibody. Overlay histogram showing PC-3 cells stained with anti-MASP1 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MASP1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to MASP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MASP1 recombinant protein (Position: R169-E580). Human MASP1 shares 84.5% and 83.3% amino acid (aa) sequence identity with mouse and rat MASP1, |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MASP1 |
|---|---|
| Gene Synonyms | 3MC1 MAP1 MASP RaRF CRARF MAP-1 MASP3 MAp44 PRSS5 CRARF1 MASP-3 |
| Gene Full Name | MBL associated serine protease 1 |
| Gene Summary | This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
| Alternative Names | Complement factor MASP 3 CRARF CRARF1 MASP MASP 1 MASP1 PRSS5 RaRF Serine protease 5 |
| Molecular Weight(MW) | 79 kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of MASP1 using anti-MASP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MASP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of PC-3 cells using anti-MASP1 antibody. Overlay histogram showing PC-3 cells stained with anti-MASP1 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MASP1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
