Application
IHC Image using - Detection of Mast cell tryptase by IHC in Human Tonsil| Product Name | Mast cell tryptase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AA1] to Mast Cell TryptaseMast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. |
| Immunogen | Purified human lung Tryptase |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AA1 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-PWB |
| IHC: This antibody may be diluted to a titer of 1:100-1:200 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | HumanDogMonkeyCat |
| Concentration | 1mg/ml |
| Gene Synonyms | TPS2 MGC104064 tryptaseB TPS1 tryptaseC TPSB1 |
|---|---|
| Target | Mast cell tryptase |
| Tissue Specificity | This antibody reacts with mast cells distributed in skin, synovium, lung and heart. It does not bind with any other cell type. |
| Cellular Localization | Cytoplasmic |
Application
IHC Image using - Detection of Mast cell tryptase by IHC in Human Tonsil| Positive Control | Tonsil |
|---|---|
| Application Notes | IHC: This antibody may be diluted to a titer of 1:100-1:200 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4-8 °C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.05% sodium azide |
