Application
Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Application
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50, ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)| Product Name | MAT2A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT3A2] to MAT2AMethionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer. |
| Immunogen | Recombinant human MAT2A (1-395aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AT3A2 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| Western Blot: Use at 1:1000 ~ 2000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Methionine adenosyltransferase MATII SAMS2 MAT II AMS 2 S adenosylmethionine synthetase isoform type 2 S adenosylmethionine synthetase gamma form Methionine adenosyltransferase II alpha MATA 2 MAT2A AMS2 S adenosylmethionine synthetase isoform t |
|---|---|
| Target | MAT2A |
| Light Chain Type | kappa |
Application
Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Application
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50, ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)| Application Notes | Western Blot: Use at 1:1000 ~ 2000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
