Application
IHC-P analysis of human heart tissue using MBD2 antibody at 1:100. Left to right:DAPI, MBD2, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.Application
WB analysis of HepG2 cells using MBD2 antibody at 0.625μg/ml. Lane A : marker Lane B : HepG2 cellsApplication
WB analysis of mouse ES cells using MBD2 antibody at 1:1000.Loading amount : 15ug. Lane 1 : MBD2 KO mouse ES cell lysate Lane 2 : Wild type mouse ES cell lysate| Product Name | MBD2 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MBD2DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human MBD2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | NY CO41 MBD2 DMTASE |
|---|---|
| Target | MBD2 |
| Molecular Weight(MW) | 43 KDa (note) |
Application
IHC-P analysis of human heart tissue using MBD2 antibody at 1:100. Left to right:DAPI, MBD2, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
Application
WB analysis of HepG2 cells using MBD2 antibody at 0.625μg/ml. Lane A : marker Lane B : HepG2 cells
Application
WB analysis of mouse ES cells using MBD2 antibody at 1:1000.Loading amount : 15ug. Lane 1 : MBD2 KO mouse ES cell lysate Lane 2 : Wild type mouse ES cell lysate| Positive Control | HepG2 , mouse ES cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
