Application
Western blot analysis of Mcl-1in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 ug/mL.Application
Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 ug/mL.Application
Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 20 ug/mL.| Product Name | MCL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MCL1This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010] |
| Immunogen | Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Mcl-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 1 - 2 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MCL1L myeloid cell leukemia 1 MCL1-ES bcl2-L-3 mcl1/EAT TM Mcl-1 MCL1S EAT BCL2L3 |
|---|---|
| Target | MCL1 |
| Tissue Specificity | Detects isoforms Mcl-1L and Mcl-1S |
Application
Western blot analysis of Mcl-1in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 ug/mL.
Application
Immunofluorescence of Mcl-1 in Raji cells with Mcl-1 antibody at 20 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
