Application
Western blot analysis of MCP-1 in LPS treated RAW cell lysate.| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MCP-1Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. The 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | NeutWB |
| Western blot analysis (0.5-2 µg/ml). From researcher’s feedback, it can also be used for neutralization studies. However, the optimal conditions should be determined individually.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MCP1 monocyte chemoattracctant protein 1 monocyte chemoattracctant protein MCP 1 monocyte chemoattracctant protein1 MCP-1 monocyte chemoattracctant protein-1 |
|---|---|
| Target | MCP-1 |
| Tissue Specificity | This antibody is specific for MCP-1. |
Application
Western blot analysis of MCP-1 in LPS treated RAW cell lysate.| Application Notes | Western blot analysis (0.5-2 µg/ml). From researcher’s feedback, it can also be used for neutralization studies. However, the optimal conditions should be determined individually.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C.The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% sodium azide |
