Application
Rat MCP-1 detected in RAW cells stimulated with LPS for 15 hrs. 1. Lysate 2. Supernatant| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MCP1Rat MCP-1/JE (monocyte chemoattracctant protein-1) is an 148 amino acid CC chemokine with a NH2- terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library1. This rat MCP-1/JE is 49 amino acid longer than human MCP-1 at 3’- end. This 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O- glycosylation and which explains for the higher molecular weight (25 kDa)2. In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP- 1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli. |
| Immunogen | Recombinant fragment - amino acids 1-73 of rat MCP-1 (N-terminal) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:1,500 - 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MCP-1 Scya2 Sigje MCP 1 MCP1 |
|---|---|
| Target | MCP1 |
Application
Rat MCP-1 detected in RAW cells stimulated with LPS for 15 hrs. 1. Lysate 2. Supernatant| Application Notes | For WB: Use at a dilution of 1:1,500 - 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.2, containing 0.1% sodium azide |
