Application
MCP-1 detected in RAW cells stimulated with LPS for 15hrs Lane1:Lysate Lane2:Supernatant| Product Name | MCP1 / CCL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MCP1Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than humanMCP-1 at 3'-end. This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli. |
| Immunogen | Recombinant full length protein (Rat) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | MCP-1 Scya2 Sigje MCP 1 |
|---|---|
| Target | MCP1 |
Application
MCP-1 detected in RAW cells stimulated with LPS for 15hrs Lane1:Lysate Lane2:Supernatant| Application Notes | WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.1% sodium azide |
