| Product Name | MCP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [542] to MCP3This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. |
| Immunogen | Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 542 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 8.5 kDa. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3μg/well Substrate:TMB.A Western blot using an antibody concentration of 0.5-2μg/mL will allow visualization of 0.1μg/lane of human MCP-3. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Monocyte chemoattractant protein3 SCYA6 MCP3 Monocyte chemotactic protein 3 CCL7 SCYA7 FIC NC28 Chemokine CC Motif Ligand 7 Monocyte chemoattractant protein 3 Chemokine CC Motif Ligand7 Small inducible cytokine A7 MARC Monocyte chemotactic pro |
|---|---|
| Target | MCP3 |
| Tissue Specificity | This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF. |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 8.5 kDa. This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3μg/well Substrate:TMB.A Western blot using an antibody concentration of 0.5-2μg/mL will allow visualization of 0.1μg/lane of human MCP-3. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 0.01M PBS. pH 7.2 |
