Application
Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.Application
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.Application
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.| Product Name | MD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to MD-2 [9F1B1]MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4. |
| Immunogen | Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 9F1B1 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MD-2 (9F1B1): Lymphocyte antigen 96 precursor MD2 (9F1B1): Lymphocyte antigen 96 precursor ESOP-1 MD2 (9F1B1): Lymphocyte antigen96 precursor ESOP1 MD2 ESOP 1 |
|---|---|
| Target | MD-2 |
| Tissue Specificity | This antibody is specific for MD-2 |
Application
Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.
Application
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
Application
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
