Application
MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MDM2 protein stained by MDM2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of mouse MDM2-transfected 293T cells 7.5 % SDS-PAGE MDM2 antibody dilution: 1:1000| Product Name | MDM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HDMX antibody MGC5370 antibody MGC71221 antibody hdm2 antibody MDM2 antibody Mdm2 transformed 3T3 cell double minute 2 p53 binding protein antibody MDM2 variant FB30 antibody double minute 2 human homolog of p53-binding protein antibody MDM2 va |
|---|---|
| Target | MDM2 |
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleoplasm , Cytoplasm , nucleolus |
Application
MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MDM2 protein stained by MDM2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of mouse MDM2-transfected 293T cells 7.5 % SDS-PAGE MDM2 antibody dilution: 1:1000| Positive Control | mouse MDM2-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
