Application
MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples: Jurkat whole cell extract A. 30 μg Jurkat cell whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MDM2 protein by 4 μg MDM2 antibody 7.5 % SDS-PAGE The immunoprecipitated MDM2 protein was detected by MDM2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody diluted at 1:1000.Application
MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2 antibody at 1:500 dilution.Application
MDM2 antibody detects MDM2 protein at cytoplasm in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. MDM2 antibody diluted at 1:500.Application
Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.| Product Name | MDM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Dog, Cat, Pig, Rhesus Monkey, BovineDog (91%), Cat (95%), Pig (93%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HDMX antibody MGC5370 antibody MGC71221 antibody hdm2 antibody MDM2 antibody Mdm2 transformed 3T3 cell double minute 2 p53 binding protein antibody MDM2 variant FB30 antibody double minute 2 human homolog of p53-binding protein antibody MDM2 va |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Nucleus , nucleoplasm , Cytoplasm , nucleolus |
Application
MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples: Jurkat whole cell extract A. 30 μg Jurkat cell whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MDM2 protein by 4 μg MDM2 antibody 7.5 % SDS-PAGE The immunoprecipitated MDM2 protein was detected by MDM2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody diluted at 1:1000.
Application
MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2 antibody at 1:500 dilution.
Application
MDM2 antibody detects MDM2 protein at cytoplasm in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. MDM2 antibody diluted at 1:500.
Application
Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.| Positive Control | H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
