Application
Immunoprecipitation of MDM-2 protein from Jurkat whole cell extracts using 5 μg of MDM2 antibody [N1N3] or MDM2 antibody . Western blot analysis was performed using MDM2 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract D. 30 µg HCT116 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg HL-60 whole cell lysate/extract F. 30 µg NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.| Product Name | MDM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Pig, Rhesus Monkey, BovineDog (92%), Cat (94%), Pig (94%), Rhesus Monkey (100%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HDMX antibody MGC5370 antibody MGC71221 antibody hdm2 antibody MDM2 antibody Mdm2 transformed 3T3 cell double minute 2 p53 binding protein antibody MDM2 variant FB30 antibody double minute 2 human homolog of p53-binding protein antibody MDM2 va |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
Application
Immunoprecipitation of MDM-2 protein from Jurkat whole cell extracts using 5 μg of MDM2 antibody [N1N3] or MDM2 antibody . Western blot analysis was performed using MDM2 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract D. 30 µg HCT116 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MDM2 antibody [N1N3] detects MDM2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg HL-60 whole cell lysate/extract F. 30 µg NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE MDM2 antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.| Positive Control | A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , JC , BCL-1 , Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , rat brain , *MCF7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
