Application
IHC-P analysis of human breast carcinoma tissue using MDM2 antibody [SMP14].| Product Name | MDM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [SMP14] to MDM2This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | SMP14 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-PIPIHC-R |
| Immunohistology - Paraffin: 1/200 - 1/500 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hdm2 ACTFS HDMX MDM2 oncogene E3 ubiquitin protein ligase |
|---|---|
| Target | MDM2 |
| Tissue Specificity | This antibody recognizes MDM2. |
Application
IHC-P analysis of human breast carcinoma tissue using MDM2 antibody [SMP14].| Application Notes | Immunohistology - Paraffin: 1/200 - 1/500 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
