Application
Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody. The picture on the right is treated with the synthesized peptide.| Product Name | MDMX (phospho Ser367) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDMX (phospho Ser367)This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011] |
| Immunogen | Peptide sequence around phosphorylation site of serine 367 (T-I-S(p)-A-P) derived from Human MDM4. |
| Modification | p-Ser367 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:3000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HDMX MRP1 MDM4 p53 regulator MDMX MDM4 |
|---|---|
| Target | MDMX (phospho Ser367) |
| Tissue Specificity | The antibody detects endogenous levels of MDM4 only when phosphorylated at serine 367. |
Application
Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | WB: 1:500 - 1:3000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
