Application
Western blot analysis of MeCP2 in HeLa cell lysate using at 2ug/ml.| Product Name | MeCP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MeCP2DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families. |
| Immunogen | Mixture of synthetic peptides EKSEDQDLQGLKDKP and APGTGRGRGRPKGSG, corresponding to aminoacids 11-25 and 181-195, respectively (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | AUTSX3 DKFZp686A24160 MRX16 MRX79 MRXS13 MRXSL PPMX RS RTS RTT |
|---|---|
| Target | MeCP2 |
| Cellular Localization | Nuclear |
Application
Western blot analysis of MeCP2 in HeLa cell lysate using at 2ug/ml.| Positive Control | HeLa cells. |
|---|---|
| Application Notes | WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
