Application
ChIP was performed with U2OS nuclear extract and either 5 μg of H3K79me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to β-Actin or Sat2.Application
WB analysis of nuclear extracts (40 μg) from HeLa cells using MeCP2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of MeCP2 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.| Product Name | MeCP2 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MeCP2 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWBChIP |
| ChIP (5 µg/ChIP), ELISA (1:1,000), Western blotting (1:1,000): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MRXS13 AUTSX3 methyl CpG binding protein 2 (Rett syndrome) MRXSL RS MECP2 RTT PPMX MRX79 RTS MRX16 |
|---|---|
| Target | MeCP2 |
Application
ChIP was performed with U2OS nuclear extract and either 5 μg of H3K79me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to β-Actin or Sat2.
Application
WB analysis of nuclear extracts (40 μg) from HeLa cells using MeCP2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of MeCP2 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.| Application Notes | ChIP (5 µg/ChIP), ELISA (1:1,000), Western blotting (1:1,000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
