Application
MED25 antibody detects MED25 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MED25 antibody diluted at 1:1000.| Product Name | MED25 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED25 (mediator complex subunit 25)This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center of human MED25. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (91%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACID1 mediator complex subunit 25 CMT2B2 ARC92 P78 PTOV2 TCBAP0758 |
|---|---|
| Target | MED25 |
| Molecular Weight(MW) | 78 kDa (note) |
| Cellular Localization | Nucleus |
Application
MED25 antibody detects MED25 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MED25 antibody diluted at 1:1000.| Positive Control | A431 , HeLa , HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
