Application
WB analysis of human heart tissue using MED25 antibody at 0.2-1μg/ml.Application
ChIP analysis of HCT116 cells using MED25 antibody. Use 0.5μg antibody MED25 antibody for ChIP assay.Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide| Product Name | MED25 antibody, N-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MED25This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human MED25 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 85%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TCBAP0758 CMT2B2 ARC92 P78 PTOV2 ACID1 MED25 |
|---|---|
| Target | MED25 |
| Molecular Weight(MW) | 78 KDa (note) |
Application
WB analysis of human heart tissue using MED25 antibody at 0.2-1μg/ml.
Application
ChIP analysis of HCT116 cells using MED25 antibody. Use 0.5μg antibody MED25 antibody for ChIP assay.Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide| Positive Control | human heart |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
