| Product Name | MEK2 (phospho Thr394) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MEK2 ( phospho T394 )Microtubule associated protein kinase kinase 2 (MEK 2 or MAPKK 2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine - glutamic acid - tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK 2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK 2. |
| Immunogen | Synthetic peptide (Mouse). |
| Modification | p-Thr394 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 48 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AA589381 MEK2 MK2 Prkmk2 MEK2 (phospho T394) MEK2 (phospho Thr394) MEK2 (pT394) MEK2 (pThr394) MEK2 phospho T394 MEK2 phospho Thr394 Phospho MEK2 (T394) Phospho MEK2 (Thr394) Phospho MEK2 (pT394) Phospho MEK2 (pThr394) |
|---|---|
| Target | MEK2 |
| Tissue Specificity | This antibody is not expected to react with MEK 1, which is 64% homologous. |
| Positive Control | A431 cells +/- EGF |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 48 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3 |
