Application
Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).Application
Figure 2:Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Western blot analysis using MAP2K2 mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.| Product Name | MEK2 antibody [7F5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7F5] to MAP2K2The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human MAP2K2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F5 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPKK2 , PRKMK2 , MKK2 , MEK2 |
|---|---|
| Target | MAP2K2 |
| Molecular Weight(MW) | 44 kDa (note) |
Application
Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).
Application
Figure 2:Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Western blot analysis using MAP2K2 mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
