Application
Western blot analysis of extracts from Rat and Mouse brain tissue using MEK5(phospho-Ser311/ Thr315) Antibody| Product Name | MEK5 (phospho Ser311/ Thr315) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK5(Phospho Ser311/ Thr315)Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis. |
| Immunogen | Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5. |
| Modification | p-Ser311/ Thr315 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MEK 5, mitogen-activated protein kinase kinase 5, MAPK/ERK kinase 5, MAPKK 5, dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, Mek5, Map2k5, mitogen activated protein kinase kinase 5 |
|---|---|
| Target | MEK5(Phospho Ser311/ Thr315) |
| Molecular Weight(MW) | 46 (note) |
Application
Western blot analysis of extracts from Rat and Mouse brain tissue using MEK5(phospho-Ser311/ Thr315) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
