Application
Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody| Product Name | MEK5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MEK5A family of protein kinases located upstream of the MAP kinases andresponsible for their activation has been identified. The prototype member ofthis family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present inthe Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to acti-vate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activatesboth p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway. |
| Immunogen | Peptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MEK 5, mitogen-activated protein kinase kinase 5, MAPK/ERK kinase 5, MAPKK 5, dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, Mek5, Map2k5, mitogen activated protein kinase kinase 5 |
|---|---|
| Target | MEK5 |
| Molecular Weight(MW) | 49 (note) |
Application
Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
