Application
IHC-P analysis of human melanoma using Melan A antibody [A103].| Product Name | Melan A antibody [A103] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [A103] to MelanAMART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | A103 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For IP: Use at a dilution of 1:50. For WB: Use at a dilution of 1:50-1:100. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | MART-1, MART1, MART 1 |
|---|---|
| Target | Melan A |
| Tissue Specificity | This antibody does not cross-react with MAGE-1 or tyrosinase protein. |
| Cellular Localization | Cytoplasmic |
Application
IHC-P analysis of human melanoma using Melan A antibody [A103].| Positive Control | Melanoma |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For IP: Use at a dilution of 1:50. For WB: Use at a dilution of 1:50-1:100. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide |
