| Product Name | Melan A antibody [DT101 + BC199] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DT101 + BC199 ] to Melan AMART-1 (Melanoma Antigen Recognized by T cells) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | DT101 + BC199 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC:For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | Antigen SK29 AA, MART1, Melan A protein, Melanoma antigen recognized by T cells 1, |
|---|---|
| Target | Melan A |
| Tissue Specificity | This antibody recognizes a protein of 18kDa, identified at Melan A (Melanoma Antigen Recognized by T- cells 1) or Melan-A. The MART-1 recognizes a subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Both HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Studies have shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas. |
| Cellular Localization | Endoplasmic reticulum and melanosomes. |
| Positive Control | Melanoma |
|---|---|
| Application Notes | IHC:For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 °C. |
| Storage Buffer | Preservative: Proprietary, not sodium azide or thimerosal. Constituents: PBS, Tissue Culture Supernatant. pH 7.3; Protein carrier. |
