Application
Formalin fixed paraffin embedded human melanoma stained with Melanoma antibody .| Product Name | Melanoma antibody [HMB45] (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HMB45] to Melanoma predilutedMalignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.[supplied by OMIM] |
| Immunogen | BALB/C mice were injected with extract of pigmented melanoma metastases from lymph nodes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HMB45 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CMM CMM1 Cutaneous Malignant Melanoma Cutaneous Malignant Melanoma Dysplastic Nevus DNS Dysplastic Nevus Syndrome FAMMM MLM |
|---|---|
| Target | Melanoma |
| Tissue Specificity | This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human melanoma stained with Melanoma antibody .| Application Notes | This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS containing 0.05% sodium azide |
