Application
Formalin fixed paraffin embedded lymph node stained with Pan Melanoma Cocktail antibodyApplication
IHC Image using - Detection of Melanoma by IHC in Human Lymph Node| Product Name | Melanoma antibody [mixture] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HMB45 ( IgG1 / k ) + M2 - 7C10 ( IgG2b ) + M2 - 9E3 ( IgG2b ) + T311 ( IgG2a )] to MelanomaThe HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. The MART-1/Melan A recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45 and the MART-1 (cocktail) and Tyrosinase make this quadruple antibody cocktail a first-order pan melanoma screener. |
| Immunogen | HMB45 - Pigmented melanoma metastases from lymphnodes. MART-1 - Recombinant human MART-1 protein. Tyrosinase - Recombinant tyrosinase protein. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | mixture |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| IHC: For an ABC system, dilute 1/25-1/50 and incubate for 30-60 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | Melanoma |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
Formalin fixed paraffin embedded lymph node stained with Pan Melanoma Cocktail antibody
Application
IHC Image using - Detection of Melanoma by IHC in Human Lymph Node| Positive Control | Metastatic melanoma in lymph node |
|---|---|
| Application Notes | IHC: For an ABC system, dilute 1/25-1/50 and incubate for 30-60 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: Tissue culture supernatant |
