Application
IHC Image using - Detection of Mesothelioma by IHC in Human MesotheliomaApplication
Formalin fixed paraffin embedded human mesothelioma stained with Mesothelioma antibody .| Product Name | Mesothelioma antibody [HBME-1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HBME - 1] to Mesothelioma |
| Immunogen | Suspension of human mesothelioma cells from patients with malignant epithelial mesothelioma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | HBME-1 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC: This antibody may be diluted to a titer of 1:12-1:25 in an ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Target | Mesothelioma |
|---|---|
| Tissue Specificity | This antibody reacts with an unknown antigen on microvilli of mesothelioma cells. It stains normal mesothelial cells as well as epithelial mesothelimias in a thick membrane pattern due to abundant lung microvilli on the surface of these cells. |
| Cellular Localization | Cell membrane. |
Application
IHC Image using - Detection of Mesothelioma by IHC in Human Mesothelioma
Application
Formalin fixed paraffin embedded human mesothelioma stained with Mesothelioma antibody .| Positive Control | Mesothelioma. |
|---|---|
| Application Notes | IHC: This antibody may be diluted to a titer of 1:12-1:25 in an ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4-8 °C. |
| Storage Buffer | PBS with sodium azide |
