Application
Flow cytometric analysis of Hela cells using anti-Metadherin mAb (green) and negative control (purple).Application
Immunohistochemical analysis of paraffin-embedded human liver cancer(A) and brain tissue(B), showing cytoplasmic localization using anti-Metadherin antibody with DAB staining.Application
Western blot analysis using anti-Metadherin mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.| Product Name | Metadherin antibody [2F11C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2F11C3] to Metadherin |
| Immunogen | The immunogen corresponding to a region within amino acids 378 and 579 of human MTDH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F11C3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LYRIC/3D3 , MTDH , 3D3 , AEG-1 , LYRIC , AEG1 |
|---|---|
| Target | Metadherin |
| Molecular Weight(MW) | 64 kDa (note) |
Application
Flow cytometric analysis of Hela cells using anti-Metadherin mAb (green) and negative control (purple).
Application
Immunohistochemical analysis of paraffin-embedded human liver cancer(A) and brain tissue(B), showing cytoplasmic localization using anti-Metadherin antibody with DAB staining.
Application
Western blot analysis using anti-Metadherin mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.| Application Notes | Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | PBS containing 50% glycerol and 0.03% sodium azide. |
