Application
ApplicationApplication
Western blot analysis of Lane 1: MW marker Lane 2: MT I proetin isolated from rabbit liver Lane 3: MT II protein isolated from rabbit liver Lane 4: Mummichug CdCl2Application
Immunohistochemistry analysis of human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10µg/ml.WB
H. Expression analysis of cell-cycle-related proteins at protein levels. Cell proteins were then extracted and the expression of CDK2, CDK4, MT1G, p-Rb and Rb were determined by Western blot. Actin was used as internal control.| Product Name | Metallothionein antibody [UC1MT] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [UC1MT] to MetallothioneinMetallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages. |
| Immunogen | Full length protein (cross linked rabbit liver MT1 and MT2). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | UC1MT |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Use at a concentration of 1 μg/ml. A band of approximately 6 kDa is detected in human, rabbit and horse samples, and a band of approximately 15 kDa is detected in mummichug samples.WB:1:500~1:1000 IHC:1:50~1:100 |
|
| Species Reactivity | FishHorseHumanMouseRabbitRat |
| Concentration | 1mg/ |
| Purification | Protein G |
| Target | Metallothionein |
|---|---|
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Application
Application
Western blot analysis of Lane 1: MW marker Lane 2: MT I proetin isolated from rabbit liver Lane 3: MT II protein isolated from rabbit liver Lane 4: Mummichug CdCl2
Application
Immunohistochemistry analysis of human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10µg/ml.
WB
H. Expression analysis of cell-cycle-related proteins at protein levels. Cell proteins were then extracted and the expression of CDK2, CDK4, MT1G, p-Rb and Rb were determined by Western blot. Actin was used as internal control.| Application Notes | Use at a concentration of 1 μg/ml. A band of approximately 6 kDa is detected in human, rabbit and horse samples, and a band of approximately 15 kDa is detected in mummichug samples.WB:1:500~1:1000 IHC:1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.2, PMSF and 50% glycerol |
