Application
WB analysis of HeLa lysate (35ug) using MINCLE antibody at a dilution of 1:1,000.Application
IHC-P analysis of colorectal cancer tissue using MINCLE antibody at a dilution of 1:50.Application
ICC/IF analysis of HeLa cells using MINCLE antibody antibody at a dilution of 1:500 (Red). Nuclei were stained by Hoechst 33342 (blue).| Product Name | MINCLE antibody [AT16E3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT16E3] to MINCLEThis gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human MINCLE (41-219aa) purified from E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AT16E3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLECSF9 , CLEC4E , C-type lectin domain family 4, member E |
|---|---|
| Target | MINCLE |
| Light Chain Type | kappa |
Application
WB analysis of HeLa lysate (35ug) using MINCLE antibody at a dilution of 1:1,000.
Application
IHC-P analysis of colorectal cancer tissue using MINCLE antibody at a dilution of 1:50.
Application
ICC/IF analysis of HeLa cells using MINCLE antibody antibody at a dilution of 1:500 (Red). Nuclei were stained by Hoechst 33342 (blue).| Application Notes | The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
