Application
Western blot analysis of MITF in A375 lysate using MITF antibody at 4 ug/ml.Application
Lane 1 : MiTF antibody [21D1418] at 1/1000 dilution Lane 2 : MiTF antibody [21D1418] at 1/200 dilution Lane 1 : A375 cell lysate Lane 2 : Raw lysate at 10 ug| Product Name | MiTF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [21D1418] to MiTFMiTF is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. |
| Immunogen | Synthetic peptide: SEARALAKERQK, corresponding to amino acids 301-312 of Human MiTF. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 21D1418 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a dilution of 1/250 - 1/1000. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRatXenopusBovine |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | MI WS2 WS2A bHLHe32 |
|---|---|
| Target | MiTF |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Western blot analysis of MITF in A375 lysate using MITF antibody at 4 ug/ml.
Application
Lane 1 : MiTF antibody [21D1418] at 1/1000 dilution Lane 2 : MiTF antibody [21D1418] at 1/200 dilution Lane 1 : A375 cell lysate Lane 2 : Raw lysate at 10 ug| Positive Control | A375 (human) and Raw (mouse) cells |
|---|---|
| Application Notes | WB: Use at a dilution of 1/250 - 1/1000. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
