Application
Immunohistochemical staining using microphthalmia antibody on formalin fixed, paraffin embedded human melanoma.| Product Name | MiTF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [C5] to microphthalmiaThis gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. |
| Immunogen | N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C5 |
| Host Species | Mouse |
| Tested Applications | GSAIHC-FIHC-PIPWB |
| For IP: Use at a concentration of 2 μg/ml. For WB: Use at a concentration of 1 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | MI WS2 WS2A bHLHe32 |
|---|---|
| Target | MiTF |
Application
Immunohistochemical staining using microphthalmia antibody on formalin fixed, paraffin embedded human melanoma.| Positive Control | 501 Mel human melanoma cells and wild-type human, rat or mouse osteoclast cells |
|---|---|
| Application Notes | For IP: Use at a concentration of 2 μg/ml. For WB: Use at a concentration of 1 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.08% sodium azide |
