Application
Immunofluorescence staining of methanol-fixed Hela cells using MKK3 (Phospho-Ser189) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3 (Phospho-Ser189) Antibody .Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) Antibody| Product Name | MKK3 (phospho Ser189) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MKK3 (Phospho Ser189)Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
| Immunogen | Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3. |
| Modification | p-Ser189 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK3 MEK3 MKK3 PRKMK3 MAP2K3 MAPK/ERK kinase 3 MEK 3 SAPK kinase 2 MAP kinase kinase 3 MAPKK 3 dual specificity mitogen-activated protein kinase kinase 3 stress-activated protein kinase kinase 2 mitogen-activated protein kinase kinase 3 |
|---|---|
| Target | MKK3 (Phospho Ser189) |
| Molecular Weight(MW) | 40 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using MKK3 (Phospho-Ser189) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3 (Phospho-Ser189) Antibody .
Application
Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
