Application
Immunofluorescence staining of methanol-fixed Hela cells using MKK3 Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from MDA cells using MKK3 Antibody .Application
Western blot analysis of extracts from MDA cells using MKK3(Ab-189) Antibody| Product Name | MKK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MKK3MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. |
| Immunogen | Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK3 MEK3 MKK3 PRKMK3 MAP2K3 MAPK/ERK kinase 3 MEK 3 SAPK kinase 2 MAP kinase kinase 3 MAPKK 3 dual specificity mitogen-activated protein kinase kinase 3 stress-activated protein kinase kinase 2 mitogen-activated protein kinase kinase 3 |
|---|---|
| Target | MKK3 |
| Molecular Weight(MW) | 40 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using MKK3 Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from MDA cells using MKK3 Antibody .
Application
Western blot analysis of extracts from MDA cells using MKK3(Ab-189) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
