| Product Name | MLH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [14] to MLH1MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 14 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| For IHC-P: Use at a dilution of 9-11 μg/ml. For WB: Use at a concentration of 1 - 1.5 μg/ml. Detects a band of approximately 83 kDa. Optimal dilutions/concentrations should be determined by the researcher. For paraffin sections, pretreat with heat for 20 minutes.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNPCC P40692 COCA2 MLH1 FCC2 4292 114030 hMLH1 MGC5172 HNPCC2 |
|---|---|
| Target | MLH1 |
| Cellular Localization | Nuclear |
| Light Chain Type | Kappa |
| Positive Control | LoVo cells or colon tissue |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 9-11 μg/ml. For WB: Use at a concentration of 1 - 1.5 μg/ml. Detects a band of approximately 83 kDa. Optimal dilutions/concentrations should be determined by the researcher. For paraffin sections, pretreat with heat for 20 minutes.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 0.05 M sodium phosphate buffer, 0.2% gelatin with =0.1% sodium azide |
