Application
Western blot analysis of MLH1 in Daudi cell lysate using at 2 µg/ml.Application
Western blot analysis of MLH-1 in Molt-4 cell lysate using at 2 g/ml, and exposed for 15 minutes.| Product Name | MLH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [164C819] to MLH1MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 164C819 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 2 - 3 μg/ml. Detects a band of approximately 83 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | HNPCC P40692 COCA2 MLH1 FCC2 4292 114030 hMLH1 MGC5172 HNPCC2 |
|---|---|
| Target | MLH1 |
| Cellular Localization | Nuclear |
Application
Western blot analysis of MLH1 in Daudi cell lysate using at 2 µg/ml.
Application
Western blot analysis of MLH-1 in Molt-4 cell lysate using at 2 g/ml, and exposed for 15 minutes.| Positive Control | Molt-4 cell lysate |
|---|---|
| Application Notes | WB: Use at a concentration of 2 - 3 μg/ml. Detects a band of approximately 83 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
