| Product Name | MMP18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MMP18This gene encodes a member of a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded protein is secreted as an inactive proprotein, which is activated upon cleavage by extracellular proteases. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013] |
| Immunogen | Synthetic peptide (Xenopus laevis) Hinge. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects bands of approximately 53 and 51 kDa, plus smaller migrating forms. MMP18 is produced at very low levels in normal, quiescent Xenopus cells and tissues. Collagenase production can be stimulated by mitogens such as TPA or TNF alpha in receptive cell lines. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Xenopus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MMP19 RASI-1 |
|---|---|
| Target | MMP18 |
| Tissue Specificity | Rabbit Anti-MMP18, Hinge Region specifically binds to MMP18 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). The antibody recognizes the pro-form and active forms of MMP18, as well as further activation/breakdown products. Anti MMP18, hinge region also recognizes non reduced MMP18. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects bands of approximately 53 and 51 kDa, plus smaller migrating forms. MMP18 is produced at very low levels in normal, quiescent Xenopus cells and tissues. Collagenase production can be stimulated by mitogens such as TPA or TNF alpha in receptive cell lines. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
