| Product Name | MMP19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP19Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. |
| Immunogen | Synthetic peptide (Human) Propeptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 58 kDa. MMP19 is not constituitively produced by most cell types it has been found in lung fibroblasts and gingival fibroblasts when stimulated with TPA, as well as breast tumor and osteosarcoma cell lines. Since cell types differ greatly in the quantity of MMP19 produced, the conditioned media may require mitogen stimulation or protein concentration to visualize the bands by immunoblotting. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 4327 MMP18 601807 Q99542 MMP19 RASI1 RASI-1 RASI 1 |
|---|---|
| Target | MMP19 |
| Tissue Specificity | Rabbit Anti MMP19, propeptide region specifically binds to MMP19 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc).This antibody recognizes the propeptide region of latent MMP19, which is shed during enzymatic activation. It does not recognize the activated forms of MMP19; thus it can be used to discriminate between the zymogen and the activated enzyme. |
| Cellular Localization | Secreted |
| Positive Control | Stimulated human fibroblasts |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 58 kDa. MMP19 is not constituitively produced by most cell types it has been found in lung fibroblasts and gingival fibroblasts when stimulated with TPA, as well as breast tumor and osteosarcoma cell lines. Since cell types differ greatly in the quantity of MMP19 produced, the conditioned media may require mitogen stimulation or protein concentration to visualize the bands by immunoblotting. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
