| Product Name | MMP19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP19Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000, this dilution is determined using stimulated human fibroblasts, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. By blotting against the reduced protein, the antibody reacts with bands at 58 kDa (zymogen), 45 kDa (active), and a series of further cleaved active forms. GTX11631 also recognizes non-reduced MMP19. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 4327 MMP18 601807 Q99542 MMP19 RASI1 RASI-1 RASI 1 |
|---|---|
| Target | MMP19 |
| Tissue Specificity | specifically binds to MMP19 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). The zymogen of MMP19 is found at 58 kDa and is activated to a 55 kDa form, a 45-47 kDa form, and then to a 28 kDa final active form. Depending on the activation method, MMP19 can be seen as a cascade of active forms. Anti-MMP19, C-terminal recognizes the pro-form and most active forms of MMP19. Since the hemopexin domain is cleaved from the smallest active form of MMP19, the antibody will not recognize this form. |
| Cellular Localization | Secreted |
| Positive Control | Human fibroblasts |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000, this dilution is determined using stimulated human fibroblasts, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. By blotting against the reduced protein, the antibody reacts with bands at 58 kDa (zymogen), 45 kDa (active), and a series of further cleaved active forms. GTX11631 also recognizes non-reduced MMP19. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
